Bee propolis is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals which is not limited to phenols and flavonoids. [1,2]. In this research propolis extract has been employed as a reducing and capping agent to afford silver nanoparticles (AgNPs) with bioactive potential . Propolis ethanolic extract was prepared by conventional method and subjected to chemical identification. The dried extract has been mixed with 1mM aqueous AgNO3 solution in 1:1 (v/v) at room temperature and stirred for 30 min, color change from yellow to dark brown was confirmed by UV-visible spectrophotometer at the range of 400-800 nm. This observation evident the reduction of Ag+ ions to Ag0 and further supported by Fourier transform infrared spectroscopy. Scanning electron microscopy (SEM) revealed the spherical shaped particles with size range of 90-95nm while X-ray diffraction (XRD) techniques confirms crystallinity of AgNPs. Furthermore, silver nanoparticles have studied for various biological activities [2,3] and showed excellent antioxidant, anti-inflammatory and antibacterial activities as compared to propolis ethanolic extract.. In vitro membrane stability potential of propolis AgNps results indicated that high concentration disrupted the RBc membrane integrity causing the leakage of hemoglobin.
Keywords Silver Nanoparticles, propolis ethanolic extract, Antioxidant, Anti-inflammatory, Antibacterial Activity, cytotoxicity
1. Ghramh, H. A., Khan, K. A., Ibrahim, E. H., & Ansari, M. J. (2019). Biogenic synthesis of silver nanoparticles using propolis extract, their characterization, and biological activities. Science of Advanced Materials, 11(6), 876-883.
2. Izzularab, B. M., Megeed, M., & Yehia, M. (2021). Propolis nanoparticles modulate the inflammatory and apoptotic pathways in carbon tetrachloride‐induced liver fibrosis and nephropathy in rats. Environmental Toxicology, 36(1), 55-66.
3. Corciova, A., Mircea, C., Burlec, A. F., Cioanca, O., Tuchilus, C., Fifere, A., & Hancianu, M. (2019). Antioxidant, antimicrobial and photocatalytic activities of silver nanoparticles obtained by bee propolis extract assisted biosynthesis. Farmacia, 67, 482-489.